| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.121298 |
| Chromosome: | chromosome 9 |
| Location: | 2685526 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g389851 | (1 of 274) IPR020683 - Ankyrin repeat-containing domain | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCGACCATGCGTGACAGTTTGGACCATA |
| Internal bar code: | GTAGGGATCCGGGTGGCCTCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 837 |
| LEAP-Seq percent confirming: | 99.4637 |
| LEAP-Seq n confirming: | 2040 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCGATTGGTTCATCTTCAT |
| Suggested primer 2: | ATTACCTGAGGGCGTCCTTT |