| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.121299 |
| Chromosome: | chromosome 10 |
| Location: | 303067 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g419950 | (1 of 5) PF00536 - SAM domain (Sterile alpha motif) (SAM_1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACTGCCAGTTCCGGGATGCACAGGTAGCC |
| Internal bar code: | TCATGGAGGCGACACAGGGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 586 |
| LEAP-Seq percent confirming: | 92.9822 |
| LEAP-Seq n confirming: | 7009 |
| LEAP-Seq n nonconfirming: | 529 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAAACCCAGGCATTAGAAA |
| Suggested primer 2: | AACGTCACGCCAAATACCTC |