| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.121321 |
| Chromosome: | chromosome 8 |
| Location: | 1633246 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g365500 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACGGACAACTCCTCACCGGTCGTGTGAGC |
| Internal bar code: | GAGGTTTATAGGTTAGGGTTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 529 |
| LEAP-Seq percent confirming: | 98.6774 |
| LEAP-Seq n confirming: | 1343 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACCACTTGCCGGAATGTAT |
| Suggested primer 2: | GAAGAAACCTTCGCAAGCAC |