Insertion junction: LMJ.RY0402.121331_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.121331
Chromosome:chromosome 4
Location:2396387
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre04.g221200 CGL109 Conserved in the Green Lineage; (1 of 1) IPR001005//IPR001965//IPR009057//IPR011011//IPR013083//IPR017877 - SANT/Myb domain // Zinc finger, PHD-type // Homeodomain-like // Zinc finger, FYVE/PHD-type // Zinc finger, RING/FYVE/PHD-type // Myb-like domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGCCGGGCTGTACGGCTTACGCTGCTATG
Internal bar code:CGGTTGAGTGCCGCTGAAAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:404
LEAP-Seq percent confirming:99.8239
LEAP-Seq n confirming:2268
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CTTTTGCTCTTCACCCAAGC
Suggested primer 2:ACGAGTTGGGTCTTGTCGTC