Insertion junction: LMJ.RY0402.121336_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.121336
Chromosome:chromosome 15
Location:1044447
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre15.g638550 CLR3,CLR20,RAP14,OPR79 RAP-domain containing protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGAATACCGATGGGCGTGGACCCCACCACA
Internal bar code:GGGAGACGACGGCGGGGAGCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:522
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:72
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CTGCCACTGCTCTTGTCACT
Suggested primer 2:GGTCTCTGAGAAGCAGGTGG