| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.121340 |
| Chromosome: | chromosome 9 |
| Location: | 1706990 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g396850 | (1 of 4) 2.5.1.3 - Thiamine-phosphate diphosphorylase / TMP pyrophosphorylase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTACCCTGTGTGTGTGTGTGTGTGTGTGT |
| Internal bar code: | CGTGCACGAAGTGAGGGCCTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 689 |
| LEAP-Seq percent confirming: | 30.8451 |
| LEAP-Seq n confirming: | 3376 |
| LEAP-Seq n nonconfirming: | 7569 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACAACCACGTTCCATTCTG |
| Suggested primer 2: | TTTGTTTCTTCGTTTTGGGG |