| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.121371 |
| Chromosome: | chromosome 3 |
| Location: | 1328932 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g150700 | (1 of 2) PTHR22904:SF312 - CARBOXYLATE CLAMP-TETRATRICOPEPTIDE REPEAT PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTAGGCCAGTGCGCCACTTGGCAGTCCC |
| Internal bar code: | TCATATCTAGTTCTTCTTCCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 430 |
| LEAP-Seq percent confirming: | 94.0299 |
| LEAP-Seq n confirming: | 63 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTTGGGAACGTATGGCTTG |
| Suggested primer 2: | ATGTGTGGCCGTGTAAGTCA |