| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.121402 |
| Chromosome: | chromosome 17 |
| Location: | 1953660 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g710800 | NFU3,NIFU3 | (1 of 3) PF01106 - NifU-like domain (NifU); iron-sulfur cluster assembly protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGGTATTAAGGGCGCGGGCCCTAGCGAG |
| Internal bar code: | TCAGCGAGCCGAATTTTGGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1097 |
| LEAP-Seq percent confirming: | 98.966 |
| LEAP-Seq n confirming: | 670 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCAATGTGTACGGCAGTC |
| Suggested primer 2: | TGCGTACTGATGGTGGACAT |