| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.121412 |
| Chromosome: | chromosome 14 |
| Location: | 2341974 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g623750 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTCCCCAGGCTCTGCGGTCCCGGGCAACG |
| Internal bar code: | GTCGGGTCCGGTATTACGGCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 175 |
| LEAP-Seq percent confirming: | 99.5468 |
| LEAP-Seq n confirming: | 659 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGCCACGGTACTCCTTGT |
| Suggested primer 2: | TGAATTCCGGAGTTTTCAGG |