| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.121415 |
| Chromosome: | chromosome 9 |
| Location: | 4076309 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g393432 | (1 of 1) IPR002421//IPR029060 - 5'-3' exonuclease, N-terminal // PIN domain-like | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGCGGCTATTTCCCCATCCACACGACCGC |
| Internal bar code: | TGCGGCGGGAGTGGGGAACGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 526 |
| LEAP-Seq percent confirming: | 94.3725 |
| LEAP-Seq n confirming: | 1895 |
| LEAP-Seq n nonconfirming: | 113 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCATCGTCACATACACAGC |
| Suggested primer 2: | GGCAGTGACACGAGATCTGA |