| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.121446 |
| Chromosome: | chromosome 17 |
| Location: | 5710348 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g738850 | (1 of 1) IPR000104//IPR003169 - Antifreeze protein, type I // GYF domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTTCAGGGGACCGTTGGCCATGGCAACC |
| Internal bar code: | GTCTCCCCCACGGACCTTTCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1021 |
| LEAP-Seq percent confirming: | 99.6732 |
| LEAP-Seq n confirming: | 2135 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTACACACGCATGCACACAC |
| Suggested primer 2: | CCAAGTCAAACTAGCGGAGC |