Insertion junction: LMJ.RY0402.121473_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.121473
Chromosome:chromosome 12
Location:6159459
Confidence (%):58
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAAGTCTGCACCTAGGACCATTGGCCAAAG
Internal bar code:CGAAGCCGGAATCGAAGAGGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:123
LEAP-Seq percent confirming:99.8557
LEAP-Seq n confirming:692
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:ACTTCAGAAACCCCACATGC
Suggested primer 2:TGCTCACTGCGTCAAATAGG