| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.121486 |
| Chromosome: | chromosome 11 |
| Location: | 2462410 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g475550 | UMM10 | (1 of 2) 2.1.1.165 - Methyl halide transferase / Methyl chloride transferase; methyl-halide synthetase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAGAAGGCATGTCCGCAGGCGCACGTCAC |
| Internal bar code: | GCGTGCTTTCGTGAAGGTGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 861 |
| LEAP-Seq percent confirming: | 99.8748 |
| LEAP-Seq n confirming: | 3190 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTGCGCATGTCTTGAGTG |
| Suggested primer 2: | ACATGAGGTTGGGAGGTGAG |