| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.121506 |
| Chromosome: | chromosome 12 |
| Location: | 6947247 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g560400 | DNJ16 | (1 of 1) PTHR24078:SF319 - DNAJ HOMOLOG SUBFAMILY C MEMBER 16; DnaJ-like protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGTCCACCGCCTTCGCTCCCCGTCCTTC |
| Internal bar code: | TTTGTTTTGGGATCCGTTTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 62 |
| LEAP-Seq percent confirming: | 99.6183 |
| LEAP-Seq n confirming: | 261 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCAGGTTTACAGGTGCAAT |
| Suggested primer 2: | AGTAGCAAGGTGTGGATGCC |