| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.121535 |
| Chromosome: | chromosome 2 |
| Location: | 6489382 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g116300 | (1 of 1) K15171 - transcription elongation factor SPT4 (SUPT4H1, SPT4) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCGAAACAGCAAAGCGTTTCTTTCCCCC |
| Internal bar code: | GAAGCCGGCGGGTGGATCCAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 592 |
| LEAP-Seq percent confirming: | 99.8944 |
| LEAP-Seq n confirming: | 3783 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACCTACTTGGACTCGCTC |
| Suggested primer 2: | TGTTGCTGGGCAGTGAGTAG |