| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.121569 |
| Chromosome: | chromosome 7 |
| Location: | 1223658 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g321300 | (1 of 3) PF03703 - Bacterial PH domain (bPH_2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGCTGTGGTAATACCGCTGTAAAACTGCA |
| Internal bar code: | GAATTCGCGTCCTGGCGCGACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 759 |
| LEAP-Seq percent confirming: | 99.3197 |
| LEAP-Seq n confirming: | 146 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATATGGTGAGTCGGGCACT |
| Suggested primer 2: | GCTCTGCCTCTCAAAATTGG |