| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.121599 |
| Chromosome: | chromosome 1 |
| Location: | 3698188 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g023750 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTCACATGTCTGCAGGGCGAGGGGCGCGT |
| Internal bar code: | CCTTCGAAGTATTAATACCCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 689 |
| LEAP-Seq percent confirming: | 99.1561 |
| LEAP-Seq n confirming: | 470 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACATGTGGCGAGCTCTTTT |
| Suggested primer 2: | TTGAGAAGGTGGGATTCTGG |