| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.121628 |
| Chromosome: | chromosome 1 |
| Location: | 3564575 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g022900 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACTCCATGCACCATGTTACACTGTGGGCG |
| Internal bar code: | GTATACCTAGGTCCACAGACCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1168 |
| LEAP-Seq percent confirming: | 99.7956 |
| LEAP-Seq n confirming: | 7325 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGCTGTATGTTCGCTGAAG |
| Suggested primer 2: | TGGGCTGGATAGCTAGTGCT |