| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.121632 |
| Chromosome: | chromosome 11 |
| Location: | 3210781 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g479500 | PRPL4 | (1 of 1) PTHR10746//PTHR10746:SF10 - 50S RIBOSOMAL PROTEIN L4 // 50S RIBOSOMAL PROTEIN L4, CHLOROPLASTIC; Chloroplast ribosomal protein L4 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATATTTGCACGCACACCCCTGGTGCAGAGC |
| Internal bar code: | CCGGTTGGTGGGGACTGAAATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 445 |
| LEAP-Seq percent confirming: | 99.6745 |
| LEAP-Seq n confirming: | 3675 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAGAAGGAGCAGGTGTGA |
| Suggested primer 2: | GTTGTCGGCGATTTTGTTTT |