| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.121639 |
| Chromosome: | chromosome 2 |
| Location: | 3842860 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095750 | ZEP1,ZXE1 | (1 of 1) 1.14.13.90 - Zeaxanthin epoxidase / Zea-epoxidase; Putative zeaxanthin epoxidase-like protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACTCGCGCCTGCTGGTACCTCTCCACCGC |
| Internal bar code: | TGCAGCTGTTTCTTATCCATCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 149 |
| LEAP-Seq percent confirming: | 99.0518 |
| LEAP-Seq n confirming: | 1567 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACACACACACACACCTCCA |
| Suggested primer 2: | CTCCCTCTCCATCTCCCTTC |