| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.121640 |
| Chromosome: | chromosome 10 |
| Location: | 5156037 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g456550 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTTGAACATGCGGGCCAGACCGCTGCTG |
| Internal bar code: | AAGGGTCAGGGTCGCAACGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 703 |
| LEAP-Seq percent confirming: | 99.7441 |
| LEAP-Seq n confirming: | 1559 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACAGTAGCGAGCACATCG |
| Suggested primer 2: | CGTAACACCCCTCATCTCGT |