Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.121652 |
Chromosome: | chromosome_1 |
Location: | 3891588 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre01.g025350 | FAP235 | Flagellar Associated Protein | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GATGGAAAGTGGGGAACACGGATGAGAAAC |
Internal bar code: | CGGGAATGCCGAGTTGGGGCGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 651 |
LEAP-Seq percent confirming: | 94.2308 |
LEAP-Seq n confirming: | 1078 |
LEAP-Seq n nonconfirming: | 66 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACCATACCCAGCAATTGAGC |
Suggested primer 2: | AGCTTCACACTTTGGTCGCT |