| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.121652 |
| Chromosome: | chromosome_17 |
| Location: | 763874 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g701450 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CTCCACGGCCTCCCGCAGCACCTCCTCAGG |
| Internal bar code: | CTGGGGGTCGCCGAAACGCTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 613 |
| LEAP-Seq percent confirming: | 58.9703 |
| LEAP-Seq n confirming: | 1867 |
| LEAP-Seq n nonconfirming: | 1299 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGTCAAGCTCGTCATTGTC |
| Suggested primer 2: | GAACTGGCCTTTAGCAGCC |