| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.121657 |
| Chromosome: | chromosome 16 |
| Location: | 7460614 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g686734 | (1 of 30) PTHR24349//PTHR24349:SF79 - SERINE/THREONINE-PROTEIN KINASE // NITROGEN NETWORK KINASE 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGATGAGCTTCCACGTGTGCTGCTGGCC |
| Internal bar code: | TTTTGGGTCCCCGTGTCGGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 558 |
| LEAP-Seq percent confirming: | 99.7805 |
| LEAP-Seq n confirming: | 1364 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAACTATCAGCGGGCAAGC |
| Suggested primer 2: | CGGTTCCTTTTGATTAGGCA |