| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.121658 |
| Chromosome: | chromosome 9 |
| Location: | 3603116 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g390208 | (1 of 781) IPR000104 - Antifreeze protein, type I | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTCCCGGCCTCCCATGCTGAAAGCTATC |
| Internal bar code: | AAATGGGTGGGTGCGGAGAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 859 |
| LEAP-Seq percent confirming: | 99.1348 |
| LEAP-Seq n confirming: | 2177 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCACACCCACCTCGTAAAG |
| Suggested primer 2: | ATGACCTCGCAGCTTTCTGT |