| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.121696 |
| Chromosome: | chromosome 16 |
| Location: | 7740903 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g691776 | (1 of 2) K03145 - transcription elongation factor S-II (TFIIS) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGTGCGGTGGCAACAACTGCAATTACAT |
| Internal bar code: | CGCTGAAGGTGCATGAAAAACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 846 |
| LEAP-Seq percent confirming: | 99.9224 |
| LEAP-Seq n confirming: | 1287 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACCAATTGATACCCCGCAT |
| Suggested primer 2: | CTTCACTCCACGTTACCCGT |