| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.121730 |
| Chromosome: | chromosome 14 |
| Location: | 2971472 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g627800 | (1 of 1) PTHR24119:SF0 - ACYL-COA-BINDING DOMAIN-CONTAINING PROTEIN 6 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACATACAAAGCTGCACCCGCAGACAGGCC |
| Internal bar code: | GATACACGCGTAAGGAGCTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 397 |
| LEAP-Seq percent confirming: | 99.866 |
| LEAP-Seq n confirming: | 745 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTAGCAAGAAGGGACAAG |
| Suggested primer 2: | TGATTCAACGTTAACCCCGT |