| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.121750 |
| Chromosome: | chromosome 7 |
| Location: | 1079823 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g319800 | (1 of 1) IPR000104//IPR009148 - Antifreeze protein, type I // Streptococcal non-M secreted SibA | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAACGGGCACAATAGCTCTTCACATCAG |
| Internal bar code: | CACAGGGTTTGTGGTTTTCGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 0 |
| LEAP-Seq percent confirming: | 12.0033 |
| LEAP-Seq n confirming: | 574 |
| LEAP-Seq n nonconfirming: | 4208 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTAAGGCCGACAACAACTT |
| Suggested primer 2: | ACAAGCCTGTGGTTTTGACC |