| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.121750 |
| Chromosome: | chromosome 7 |
| Location: | 1079945 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g319800 | (1 of 1) IPR000104//IPR009148 - Antifreeze protein, type I // Streptococcal non-M secreted SibA | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGAAGTGTGCACCGGCCCGCGGCACAGT |
| Internal bar code: | AGAGGACTCGTGGGGGGCTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 817 |
| LEAP-Seq percent confirming: | 99.4274 |
| LEAP-Seq n confirming: | 6598 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGAGGGGAAAGTAGCGGAG |
| Suggested primer 2: | ACAAGCCTGTGGTTTTGACC |