| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.121764 |
| Chromosome: | chromosome 12 |
| Location: | 7271089 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g557850 | (1 of 7) IPR000048//IPR027417 - IQ motif, EF-hand binding site // P-loop containing nucleoside triphosphate hydrolase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAACGTAAGGTCCCCCTGTGACCGGCGGAG |
| Internal bar code: | GGCTGTAAGGATAACCCAATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 445 |
| LEAP-Seq percent confirming: | 85.3804 |
| LEAP-Seq n confirming: | 1863 |
| LEAP-Seq n nonconfirming: | 319 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAACCCTTTATCCCACTCA |
| Suggested primer 2: | CGTAGTCGTGAGCAGCATGT |