| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.121777 |
| Chromosome: | chromosome 3 |
| Location: | 3445701 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g167250 | (1 of 1) PF05673 - Protein of unknown function (DUF815) (DUF815) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAATTCCCGCTGACCCGCCCACCTCCCCT |
| Internal bar code: | ACCGTTGTGGCGCCGTAAGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 825 |
| LEAP-Seq percent confirming: | 99.63 |
| LEAP-Seq n confirming: | 8079 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACCGTCCCTTATAGCCACA |
| Suggested primer 2: | GAGCTTTCGGGTGTGCTTAG |