| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.121785 |
| Chromosome: | chromosome 3 |
| Location: | 8139182 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g200650 | CHI2 | (1 of 1) K17525 - chitinase domain-containing protein 1 (CHID1); Chitinase-like hydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCGGTACAGGTTTCGGGGGTTGGCATTG |
| Internal bar code: | CGTATTATGAACAATCATTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 157 |
| LEAP-Seq percent confirming: | 10.0 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 90 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAGCTTCTGTGTGTGTCCG |
| Suggested primer 2: | CTGCCTTGTCGGGATACATT |