Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.121799 |
Chromosome: | chromosome 8 |
Location: | 4744516 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre08.g384250 | SNRK2O | (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase; snRK1 family in Chlamydomonas, subgroup 2 | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGCCACCGCATCGGCCTGTCCAACCGGG |
Internal bar code: | TTTTATCCGGGATCGGGCAGGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 805 |
LEAP-Seq percent confirming: | 99.685 |
LEAP-Seq n confirming: | 633 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTGTAGGTAGGTGGGTGCGT |
Suggested primer 2: | GTGCGACTTGGACGGTATTT |