| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.121806 |
| Chromosome: | chromosome 9 |
| Location: | 2517573 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g391000 | GT90F14,GT90-14 | (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90); GT90 family protein 14 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGAACGTCCGCTCCAGGTGCGCGATCATC |
| Internal bar code: | CAGGGAAGACTGAATATCTCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 303 |
| LEAP-Seq percent confirming: | 99.925 |
| LEAP-Seq n confirming: | 1333 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGAGTCCACCGGAAGTAAC |
| Suggested primer 2: | TTCCTACGTCGGGATAATCG |