| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.121833 |
| Chromosome: | chromosome 17 |
| Location: | 3403244 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g724100 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGATCCCTCGCATGCCTCGAGCCTTGTTG |
| Internal bar code: | CTAAAGGGGTCCGTGCCACAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 914 |
| LEAP-Seq percent confirming: | 93.4275 |
| LEAP-Seq n confirming: | 3440 |
| LEAP-Seq n nonconfirming: | 242 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCAAACATTTTTGGCTTGT |
| Suggested primer 2: | ACGGGTGTATTGCAGAGGAG |