| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.121852 |
| Chromosome: | chromosome 14 |
| Location: | 1663934 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g619133 | SDH1 | Succinate dehydrogenase flavoprotein subunit; (1 of 1) K00234 - succinate dehydrogenase (ubiquinone) flavoprotein subunit (SDHA, SDH1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTTCTTAACTTGTGCATGTGTGGTTTGA |
| Internal bar code: | GTGGAACAGGTTGCACATCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 817 |
| LEAP-Seq percent confirming: | 99.9078 |
| LEAP-Seq n confirming: | 2168 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTTGTGTCGGTACAGGGA |
| Suggested primer 2: | ATCTCACGCAGCACCTTCTT |