| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.121861 |
| Chromosome: | chromosome_12 |
| Location: | 8385470 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g549000 | PHC4 | Cell wall protein pherophorin-C4 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GATGCCAATTCAACTTACATTGCCGCTAGC |
| Internal bar code: | GGGGAGCATGCCGCTGACTAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 255 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 284 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTAGACCCGACAGCTTGAG |
| Suggested primer 2: | CGCTGCTATCTGCACCAATA |