Insertion junction: LMJ.RY0402.121896_3

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.121896
Chromosome:chromosome 6
Location:3529197
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g278110 (1 of 22) PTHR11017//PTHR11017:SF145 - LEUCINE-RICH REPEAT-CONTAINING PROTEIN // SUBFAMILY NOT NAMED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCTGGTTTCTGACCCAACGGCCACCGGAA
Internal bar code:AAAAGTTCACTGTTTGCGCTAC

Confirmation - LEAP-Seq

LEAP-Seq distance:1000
LEAP-Seq percent confirming:99.6825
LEAP-Seq n confirming:5966
LEAP-Seq n nonconfirming:19
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:TACTCGCAGCGAAACAACAC
Suggested primer 2:GATCCCAGCTACCACACCAC