| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.121918 |
| Chromosome: | chromosome 6 |
| Location: | 258741 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g250800 | CRB1 | (1 of 1) K13210 - far upstream element-binding protein (FUBP); C1, subunit of the circadian RNA-binding protein CHLAMY1 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAGACGTGCTATGTCTTCTTATATAGGAA |
| Internal bar code: | GAGGGTGCGACGGCTGAGCTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 502 |
| LEAP-Seq percent confirming: | 99.5192 |
| LEAP-Seq n confirming: | 414 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGTGGTCCACCTGAATCC |
| Suggested primer 2: | GTACGATACGGCATGCACAG |