| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.121930 |
| Chromosome: | chromosome 13 |
| Location: | 279318 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g564000 | GPM3,PGM3 | Phosphoglycerate mutase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTGGTCTAGGCCTCCCGTGGAGTTGCCAG |
| Internal bar code: | ACGGGGGGAGGGAGGAGCAGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 535 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 218 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACATACTCCCGAAATGCG |
| Suggested primer 2: | GCTGGGGTCGTACTTGATGT |