| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.121932 |
| Chromosome: | chromosome_6 |
| Location: | 3522854 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g278109 | Oleate/fatty acid desaturase, delta-12 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGCGCCTTCCAGACAGCCCCGCGCGCGCAT |
| Internal bar code: | AATTTGTCAGCTCCCTTTACCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 481 |
| LEAP-Seq percent confirming: | 99.3304 |
| LEAP-Seq n confirming: | 890 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAAGCTGAGTGTGCAACAT |
| Suggested primer 2: | AAGGTGAAGACCGACCACAC |