Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.121932 |
Chromosome: | chromosome_6 |
Location: | 3976378 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre06.g278194 | FAP236 | Flagellar Associated Protein | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GGGGCTGAGACGATCAGGCCTTTTAATAGC |
Internal bar code: | GACGGTCACGGTCATTAGCGGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 849 |
LEAP-Seq percent confirming: | 97.2141 |
LEAP-Seq n confirming: | 3001 |
LEAP-Seq n nonconfirming: | 86 |
LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCAACAACATTCCATACCCC |
Suggested primer 2: | AAAGCCGATAGTGGTTGTGG |