| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.121940 |
| Chromosome: | chromosome 17 |
| Location: | 491704 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g699300 | (1 of 60) 5.2.1.8 - Peptidylprolyl isomerase / Rotamase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCATGAAAGCCCCAAATGTGAATGCGTGTC |
| Internal bar code: | ACAGGGGTGGGCGGATCATCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 664 |
| LEAP-Seq percent confirming: | 85.8586 |
| LEAP-Seq n confirming: | 85 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGACGTAGGTGGCTGTCT |
| Suggested primer 2: | AGCCCACTCATGTTCTCCAC |