| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.121941 |
| Chromosome: | chromosome 16 |
| Location: | 4280776 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g668100 | SMM48 | (1 of 1) PTHR22809//PTHR22809:SF1 - METHYLTRANSFERASE-RELATED // METHYLTRANSFERASE-LIKE PROTEIN 6; S-adenosyl-L-methionine-dependent methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGTTGCCCAAGAGATGGAGTCACGGATTT |
| Internal bar code: | GAGCGGGTACGATGTAGGGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 900 |
| LEAP-Seq percent confirming: | 99.639 |
| LEAP-Seq n confirming: | 1932 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGGTTCTGTAAAACAGCGA |
| Suggested primer 2: | CGTGAGTGGGATGACAACAC |