| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.121966 |
| Chromosome: | chromosome 3 |
| Location: | 8240608 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g199600 | BES2 | (1 of 10) PF01062 - Bestrophin, RFP-TM, chloride channel (Bestrophin); putative chloride channel | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTTGAAGATGCGCTCGCAGCCGCCAGCC |
| Internal bar code: | CAAGCAAGCCTGCGCCCCGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 360 |
| LEAP-Seq percent confirming: | 99.3774 |
| LEAP-Seq n confirming: | 9418 |
| LEAP-Seq n nonconfirming: | 59 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATATGTCGTACAGGGGCAGC |
| Suggested primer 2: | AGTTCCCCCTTCACTCCACT |