| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.121969 |
| Chromosome: | chromosome 5 |
| Location: | 437331 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g232600 | (1 of 40) 3.4.24.38 - Gametolysin / Lysin | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACGAGCTTAGCTGGTGTTGAGAGTCTTTT |
| Internal bar code: | GCGGGCGTGCCGGCATAGGAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 211 |
| LEAP-Seq percent confirming: | 11.0363 |
| LEAP-Seq n confirming: | 82 |
| LEAP-Seq n nonconfirming: | 661 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACAATAATGCATTGCGAGG |
| Suggested primer 2: | GGTTGTGGAAGCTGGTGATT |