| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.121990 |
| Chromosome: | chromosome 4 |
| Location: | 2568370 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g222500 | BICD1 | Bicaudal D | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATTATGCCACAACCAAACCCTTGCTAGGA |
| Internal bar code: | CCTGGTGTCCGACAGACACCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 993 |
| LEAP-Seq percent confirming: | 99.6896 |
| LEAP-Seq n confirming: | 1606 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTCCCCAGACTCAAGCAC |
| Suggested primer 2: | GCAGTAGGGTGGGTAGGACA |