| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.122020 |
| Chromosome: | chromosome 5 |
| Location: | 2002037 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g241647 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTATCCCGTGGGTCACATACCGTGAATCGA |
| Internal bar code: | GGTGAAACCGGCGAGCAATGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 108 |
| LEAP-Seq percent confirming: | 99.5246 |
| LEAP-Seq n confirming: | 628 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTCTAGTCGTTCTGGCCG |
| Suggested primer 2: | GCTGCATGTGGCTGATCTTA |