| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.122035 |
| Chromosome: | chromosome 12 |
| Location: | 4445015 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g521336 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAGGATCTCACGCACGACTTGCGCTCGCT |
| Internal bar code: | CTGCAAAACCTGCTTATCGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 896 |
| LEAP-Seq percent confirming: | 99.375 |
| LEAP-Seq n confirming: | 3975 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATAGGGCAGCACAAGGTCAC |
| Suggested primer 2: | CAAGTTAACCCTTACCCGCA |