Insertion junction: LMJ.RY0402.122051_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.122051
Chromosome:chromosome 11
Location:3136481
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre11.g479250 RANGAP1 RAN GTPase-activating protein 1; (1 of 1) K14319 - Ran GTPase-activating protein 1 (RANGAP1) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGGATAGTTCGCGCCAGTACGACACTTTT
Internal bar code:GTCAGCACCGTTGGCTGTGACC

Confirmation - LEAP-Seq

LEAP-Seq distance:745
LEAP-Seq percent confirming:99.8092
LEAP-Seq n confirming:1046
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:ACAGGGTCGTGTTCAAGGAC
Suggested primer 2:ACAACATGGAGGAGGACGAC